other:Article Title: Highly metastatic claudin-low mammary cancers can originate from luminal epithelial cells
Article Snippet: $-TWIST; Santa Cruz; sc81417: Validated by Santa Cruz (https://www.scbt.com/p/twist-antibody-twist2c1a).
Control:Article Title: IL-17RA signaling provides dual tumor-suppressor function during late-stage colorectal carcinogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies b-Actin Sigma-Aldrich Cat# A4700; RRID:AB_476730 Gapdh Santa Cruz Cat# sc32233; RRID:AB_627679 Twist Santa Cruz Cat# sc81417; RRID:AB_1130910 pSrc y416 Cell Signaling Cat# 6943; RRID:AB_10013641 pSrc y527 Cell Signaling Cat# 2105;RRID:AB_331034 Src Cell Signaling Cat# 2109; RRID:AB_2106059 EGFR Santa Cruz Cat# sc-03; RRID:AB_631420 pSyk Cell Signaling Cat# 2710;RRID:AB_2197222 Syk Cell Signaling Cat# 13198; RRID:AB_2687924 nVivoMAb anti-mouse PD-1 Hoelzel Cat# BE0146; RRID:AB_10949053 InVivoMab mouse IgG2b isotype control Hoelzel Cat# BE0086; RRID:AB_1107791 Fixable Viability Dye-eF780 eBioscience Cat# 65-0865-14 aCD45-FITC eBioscience Cat# 11-0451-85; RRID:AB_465051 aCD11b-BV650 BD Cat# 563402; RRID:AB_2738184 aCD11c-BV785 BD Cat# 563735; RRID:AB_2738394 aLy6G-BV605 BD Cat# 563005; RRID:AB_2737946 aLy6C-AF700 Biolegend Cat# 128024; RRID:AB_10643270 aF4/80-PE eBioscience Cat# 12-4801-82; RRID:AB_465923 aPD-L1-PECy7 eBioscience Cat# 25-5982-80; RRID:AB_2573508 aCD45-BV786 BD Cat# 564225; RRID:AB_2716861 aCD4-BUV496 BD Cat# 564667; RRID:AB_2722549 aCD8-BV605 Biolegend Cat# 100744; RRID:AB_2562609 aPD1-APC eBioscience Cat# 17-9985-82; RRID:AB_11149358 aTIM-3-PECy7 Biolegend Cat# 119715; RRID:AB_2571932 aCTLA4-PE Biolegend Cat# 106305; RRID:AB_313254 aLag3-BV421 Biolegend Cat# 125221; RRID:AB_2572080 aCD4-FITC BD Cat# 553650; RRID:AB_394970 aCD8-APC eBioscience Cat# 17-0081-83; RRID:AB_469336 aCD44-AF400 Biolegend Cat# 103026; RRID:AB_493713 aCD62L-BV785 Biolegend Cat# 104440; RRID:AB_2629685 aSca-1-PerCP-Cy5.5 eBioscience Cat# 45-5981-82; RRID:AB_914372 aCD95-PE BD Cat# 554258; RRID:AB_395330 aCD8-BV650 BD Cat# 563234; RRID:AB_2738084 aCD4-BUV496 BD Cat# 564667; RRID:AB_2722549 aCD127-APC eBioscience Cat# 17-1271-82; RRID:AB_469435 aCD80-APC Invitrogen Cat# 104714; RRID:AB_313135 aCD95-FITC Biolegend Cat# 152605; RRID:AB_2632900 aIDO-AF647 BioLegend Cat# 654004; RRID:AB_2564585 aCD25-PerCP-Cy5.5 BD Cat# 561112; RRID:AB_10563212 aTCF1-PE BD Cat# 564217; RRID:AB_2687845 aCyclinD1-PE Invitrogen Cat# MA5-28534; RRID:AB_2745493 aCD8-PeCy7 Biolegend Cat# 100722; RRID:AB_312761 aCD4-AF700 Biolegend Cat# 100429; RRID:AB_493698 aGranzyme B-FITC Biolegend Cat# 515403; RRID:AB_2114575 (Continued on next page) e1 Immunity 58, 701–715.e1–e8, March 11, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER aIFNg-PerCP-Cy5.5 eBioscience Cat# 45-7311-82; RRID:AB_1107020 aTNFa-BV711 BD Cat# 563944; RRID:AB_2738499 aEGFR-PE BioLegend Cat# 352904; RRID:AB_10896794 Biological samples APTK mouse tumor organoids Nicolas et al.49 N/A APKN mouse tumor organoids Varga et al.21 N/A Human CRC organoids Farin et al.32 N/A Tp53DIEC mouse tumor organoids This study N/A Tp53DIEC/Il17ra-/-mouse tumor organoids This study N/A Tp53DIEC/Il17raDIEC mouse tumor organoids This study N/A Chemicals, peptides, and recombinant proteins Advanced DMEM F12 ThermoFisher Scientific Cat# 12634010 Fetal bovine serum Thermo Fisher Scientific Cat# 10500056 Accutase cell dissociation reagent ThermoFisher Scientific Cat# A1110501 Glutamax Invitrogen Cat# 35050038 HEPES Invitrogen Cat# 15630056 Penicillin/ Streptomycin Invitrogen Cat# 15140122 B-27 supplement ThermoFisher Scientific Cat# 12587010 N-2 supplement ThermoFisher Scientific Cat# 17502048 N-Acetylcysteine Sigma-Aldrich Cat# A9165 SB202190 BioTrend Cat# HY-10295 Human EGF PeproTech Cat# AF-100-15 Matrigel Corning Cat# 356231 Collagenase A Sigma-Aldrich Cat# C0130 Dispase II Sigma-Aldrich Cat# D4693 DNase Roche Cat# 4716728001 Y27632 Sigma-Aldrich Cat# SCM075 Mouse EGF PeproTech Cat# 315-09 TGF-b R&D Systems Cat# 7666-MB Erlotinib Absource Cat# S1023 KX2-391 Absource Cat# S2700 A83-01 Tocris Cat# 2939 EDTA ThermoFisher Scientific Cat# AM9260G Puromycin dihydrochloride Merck Cat# 58-58-2 SuperScriptII Reverse Transcriptase Invitrogen Cat# 18064-014 SYBR Green Master Mix Roche Cat# 04913914001 OligoDT Invitrogen Cat# N8080128 DNTP Invitrogen Cat# AM8200 Ribonuclease inhibitor RNAseOUT Invitrogen Cat# 10777019 Qiagen RNeasy Mini kit Qiagen Cat# 74106 Pierce Coomassie Plus (Bradford) Assay Reagent Thermo Fisher Scientific Cat# 23236 Phorbol 12-myristate 13-acetate Sigma-Aldrich Cat# P8139 Ionomycin Sigma-Aldrich Cat# I3909 Azoxymethane Sigma-Aldrich Cat# A5486 Brefeldin A BioLegend Cat# 420601 Live/Dead Fixable Blue Dead Cell Stain Kit Thermo Fisher Scientific Cat# L23105 Transcription factor buffer set BD Biosciences Cat# 562574 Cytofix/Cytoperm BD Biosciences Cat# 554714 RPMI 1640 Sigma-Aldrich Cat# R0883 (Continued on next page) Immunity 58, 701–715.e1–e8, March 11, 2025 e2
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